Accession Number:

ADA181652

Title:

Human Immune Responses to Dengue Viruses.

Descriptive Note:

Annual rept. Aug 85-Jun 86,

Corporate Author:

MASSACHUSETTS UNIV MEDICAL CENTER WORCESTER MA

Personal Author(s):

Report Date:

1986-07-01

Pagination or Media Count:

23.0

Abstract:

We have analyzed the interaction between the peripheral blood lymphocytes PBL of non-immune donors and dengue virus-infected cells, which results in interferon IFN production. Autologous monocytes or the Epstein-Barr virus transformed B lymyphoblastoid cell line Raji cells infected with dengue virus were used as IFN inducer cells. PBL produced IFN when cultured with dengue virus-infected cells. IFN was detected as early as 2 to 4 hours after exposure of PBL to dengue virus-infected cells. Dengue virus-infected cells treated with glutaraldehyde or paraformaldehyde, which produced no infectious dengue virus, also induced IFN. These results indicate that BPL produced IFN in response to dengue virus-infected cells and that the production of IFN by PBL is due to stimulation of PBL by dengue-virus-infected cells. The ability of dengue-infected cells to induce IFN correlated with the appearance of dengue antigens in infected cells. Characterization of IFN-producing PBL with monoclonal antibodies demonstrated that the IFN-producing cells were heterogeneous.

Subject Categories:

  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE