Human Immune Responses to Dengue Viruses.
Annual rept. Aug 85-Jun 86,
MASSACHUSETTS UNIV MEDICAL CENTER WORCESTER MA
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We have analyzed the interaction between the peripheral blood lymphocytes PBL of non-immune donors and dengue virus-infected cells, which results in interferon IFN production. Autologous monocytes or the Epstein-Barr virus transformed B lymyphoblastoid cell line Raji cells infected with dengue virus were used as IFN inducer cells. PBL produced IFN when cultured with dengue virus-infected cells. IFN was detected as early as 2 to 4 hours after exposure of PBL to dengue virus-infected cells. Dengue virus-infected cells treated with glutaraldehyde or paraformaldehyde, which produced no infectious dengue virus, also induced IFN. These results indicate that BPL produced IFN in response to dengue virus-infected cells and that the production of IFN by PBL is due to stimulation of PBL by dengue-virus-infected cells. The ability of dengue-infected cells to induce IFN correlated with the appearance of dengue antigens in infected cells. Characterization of IFN-producing PBL with monoclonal antibodies demonstrated that the IFN-producing cells were heterogeneous.
- Medicine and Medical Research