Human Immune Responses to Dengue Viruses.
Annual rept. Aug 84-Jul 85,
MASSACHUSETTS UNIV MEDICAL SCHOOL WORCESTER
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Peripheral blood lymphocytes PBL from humans without antibodies to dengue 2 virus lysed dengue virus-infected Raji cells to a significantly greater degree than uninfected Raji cells. Addition of mouse anti-dengue antibody increased the lysis of dengue-infected Raji cells by PBL. These results indicate that both PBL-mediated cytotoxicity and antibody-dependent cell-mediated cytotoxicity ADCC can cause significant lysis of dengue-infected cells. The effector cells responsible for lysis of dengue virus-infected Raji cells in the NK and ADCC assays were analyzed. The PBL active in lysing dengue virus-infected Raji cells were characterized using monoclonal antibodies and were compared to lymphocytes that lysed K562 cells. We have initiated studies concerning interferon production by peripheral blood mononuclear cells PBMC after infection with dengue virus. Dengue virus-infected monocytes produced IFN alpha. Dengue virus-infected monocytes induced IFN alpha from autologous PBL. To determine whether the levels of IFN which were detected could prevent dengue virus infection, monocytes were treated with 400 Uml of IFN alpha before infection. Treatment of monocytes with IFN alpha decreased the yield of infectious virus more than 99 and the percentage of dengue-antigen positive cells by 98. These results suggest that IFN produced by dengue virus-infected monocytes and PBL may have an important role in controlling dengue virus infection.
- Medicine and Medical Research