Isolation of Japanese Encephalitis Virus From Clinical Specimens Using a Continuous Mosquito Cell Line,
ARMED FORCES RESEARCH INST OF MEDICAL SCIENCES APO SAN FRANCISCO 96346
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During the 1983 Japanese encephalitis JEepidemic in northern Thailand, we systematically attempted to isolate JE virus JEV from clinical specimens collected from 49 consecutive JE patients at 1 provincial hospital. Fresh acute plasma and cerebrospinal fluid CSF samples and postmortem brain samples were immediately inoculated onto cultured monolayers of Aedes pseudoscutellaris LSTM-AP-61cells which had been shipped to the epidemic site. None of 49 plasma samples yielded virus. None of 30 fresh CSF samples from nonfatal cases yielded virus, but 5 of 15 33 CSF samples from fatal cases did. Inoculation of fresh brain specimens obtained at autopsy yielded virus in every case attempted 7 of 7, whereas postmortem needle biopsy specimens of brain yielded virus in only 1 of 4 cases. Isolates were most frequently successful using thalamic tissue 6 of 7 cases, but isolates were also commonly obtain from frontal cortex 47, occipital cortex 47, cerebellum 47 and pons 27.