Screening of Immunoenhancing Drugs with Antiviral Activity against Members of the Arena-, Alpha-, and Adenoviridae.
Annual rept. 15 Jan 86-14 Jan 87,
ALABAMA UNIV UNIVERSITY DEPT OF MICROBIOLOGY
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A series of 13 immunomodulatory compounds or mixtures of compounds have been screened for antiviral activity with either Venezuelan equine encephalitis virus, guinea pig adapted Pichinde virus, or a lung tropic plaque purified variant of mouse adenovirus as the challenge virus. Only Bordetella pertussis vaccine BPV and a Boivin extract of Bordetella pertussis EP-LPS were found to give consistent protection against a virus. The protective effect of both was seen only against adenovirus. BPV treatment appeared to result in the prolongation of life of guinea pigs challenged with Pichinde as compared to controls. CL246,738 treated mice showed a prolongation of life over buffer treated controls when challenged with Venezuelan equine encephalitis virus. The effect of EP-LPS on the immune status of injected animals was investigated in an attempt to identify the critical change that resulted in protection. EP-LPS injection did not result in any dramatic changes in the immunologic status of the treated animal. However, there was a slight decrease in the number of antibody producing cells found in the spleen after treated animal were immunized with sheep red blood cells. Further, EP-LPS treated animals showed an increased in natural killer cell activity in the spleen as compared to either buffer or E. coli LPS treated controls.