Immunologic and Genetic Selection of Adenovirus Vaccine Strains: Synthesis and Characterization of Adenovirus Antigens.
Annual rept. Jan 81-Jan 82,
COLUMBIA UNIV NEW YORK COLL OF PHYSICIANS AND SURGEONS
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Fifty conditionally lethal, temperature-sensitive ts mutants of the type 7 adenovirus vaccine strain have been isolated and characterized. Genetic analyses indicate that forty-nine of the mutants could be divided into 10 complementation groups. Selected mutants from each group were localized on the adenovirus genome by recombination and marker rescue analyses. It was striking that only one mutation involved an early gene product, the DNA-binding protein. The IIIa protein mutants are potentially excellant candidates for use in an attenuated, live virus vaccine since it produces large amounts of hexons which is the primary antigen responsible for neutralizing antibodies. Indeed, these mutants produce as much immunologically reactive hexons, fiber, and penton base as does wild-type 7 adenvirus. This contract period was devoted to characterization of these mutants in greater detail, particularly H7ts88. Keywords antiviral agents capsid proteins.