Accession Number:

ADA179898

Title:

Molecular Basis of Paralytic Neurotoxin Action on Voltage-Sensitive Sodium Channels

Descriptive Note:

Annual rept. 15 Sep 1985-14 Sep 1986

Corporate Author:

WASHINGTON UNIV SEATTLE DEPT OF PHARMACOLOGY

Personal Author(s):

Report Date:

1986-10-14

Pagination or Media Count:

48.0

Abstract:

In years 1 and 2 of this project, progress was made on several objectives The sites and mechanisms of action on the sodium channel were examined and further defined for three new classes of neurotoxins Goniopora toxins, Brevetoxins, and Conotoxins. Monoclonal antibodies with high affinity for the mammalian neuronal sodium channel were developed and methods to screen them for activity at neurotoxin binding sites were established. Site-directed antibodies against defined regions of the amion acid sequence of the sodium channel were prepared and shown to bind at discrete negatively charged subsites on the extracellular surface of the channel that may form part of neurotoxin receptor sites. Keywords Ion transport.

Subject Categories:

  • Toxicology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE