Development of a DNA-Based Method for Distinguishing the Malaria Vectors, Anopheles gambiae From Anopheles arabiensis.
Annual rept. 15 Jun 85-14 Jun 86,
EMORY UNIV ATLANTA GA DEPT OF BIOLOGY
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The Anopheles gambiae complex includes six morphologically identical species, two of which A. gambiae and A. arabiensis are the primary African malaria vectors today. Since two or more of the species are commonly sympatric, epidemiological studies to determine the involvement of each in malaria transmission have been difficult. This report describes our efforts to identify a DNA segment from A. gambiae which when used as a probe on Southern blots containing both A. gambiae and A. arabiensis DNA will reveal a restriction fragment length polymorphism. These diagnostic bands are consistently seen on blots of whole genomic DNA. The method can also be used on dried single mosquitoes or mosquito abdomens although the DNA extraction method currently used does not always yield sufficient DNA for the test. Keywords Clones Assaying Culicidae Autoradiography Hybridization.
- Medicine and Medical Research