Isolation and Partial Characterization of Pyridoxal 5'-Phosphate Hemoglobins by High-Performance Liquid Chromatography as a Quality-Control Method for Hemoglobin-Based Blood Substitutes,
DEFENCE AND CIVIL INST OF ENVIRONMENTAL MEDICINE DOWNSVIEW (ONTARIO)
Pagination or Media Count:
Pyridoxal 5 -phosphate hemoglobin PLP-Hb, prepared from hemoglobin and a four-fold excess of pyridoxal 5 -phosphate by the method of De Venuto and Zegna, has been chromatographically resolved into six components via a quaternary ammonium monobead support. On an analytical scale, the separations have been found to be rapid ca. 50 min and highly reproducible. The results also indicate that the preparation of PLP-Hb yields a reproducible product ratio. The potential of the analytical method for the routine quality control of blood substitutes derived from PLP-Hb is discussed. All five of the PLP derivatives components II-VI, isolated and purified via a combination of conventional and preparative monobead anion-exchange chromatography, gave single peaks when analyzed by high-performance liquid chromatography. Keywords plasma reprints.
- Organic Chemistry