Accession Number:

ADA179683

Title:

The Role of Chemical Inhibition of Gap-Junctional Intercellular Communication in Toxicology

Descriptive Note:

Annual technical rept. 25 Feb 1986-15 Feb 1987

Corporate Author:

MICHIGAN STATE UNIV EAST LANSING DEPT OF PEDIATRICS/HUMAN DEVELOPMENT

Personal Author(s):

Report Date:

1987-02-24

Pagination or Media Count:

12.0

Abstract:

To achieve the goal of understanding the basic mechanism of chemical inhibition of gap junctional communication a method by which cells transfer chemical information we have performed research on a validation of the fluorescence recover after photobleaching technique to study the effect of neurotoxins on cell-cell communication in living rat and human brain cells in culture b development of a new in vitro assay to measure cell-cell communication in vitro, utilizing scrape-loading and transfer of fluorescent dyes c further validation of the metabolic cooperation assay to detect tumor promoters and neurotoxins d started experiments to study the possible role of protein kinase C in the mechanism of inhibiting cell-cell communication and e the development of a new quantitative technique to measure cell communication using flow cytometry and scape-loadingdye transfer. The results described in this report have been communicated at several meetings. The abstracts are attached to the progress report. Reprints and preprints are also enclosed.

Subject Categories:

  • Toxicology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE