Accession Number:

ADA179574

Title:

Development of Rapid Methods to Monitor Oxidative and Maillard (Sugar-Amine) Polymerization in Energy-Dense, Encapsulated Model Ration Systems

Descriptive Note:

Final technical rept. Oct 1984-Oct 1985

Corporate Author:

ARMY NATICK RESEARCH DEVELOPMENT AND ENGINEERING CENTER MA

Report Date:

1986-09-01

Pagination or Media Count:

50.0

Abstract:

Energy-dense model systems containing lactose as carbohydrate, casein as protein, and soy-lecithin encapsulated corn oil as lipid have been monitored during storage at high temperatures for polymerization by means of chemical and spectrophotometric methods, including both absorption and fluorescene. The objective was to develop rapid, labor-saving methods applicable to both soluble and heavily cross-linked, insoluble components of the model systems. Systems developed to monitor Maillard browning include front-face fluorescence of acid- precipitated casein slurries, fluorescence and 410 nm absorption of the aqueous phase after pronase digestion and low intensity fluorescence of the chloroform- methanol extract of browned material. Lipid oxidation methods developed include vapor phase detection by means of polyamide plate fluorescence in presence of oxidizing lipid, front-face fluorescence, and high intensity fluorescence of the cholorform-methanol extract. The chloroform-methanol and front-face florescence methods in both lipid oxidation and Maillard browning and the polyamide method in lipid oxidation are relatively free from interference arising from common food components. They are potentially widely applicable and susceptible to semiautomation. Keywords Absorption spectra.

Subject Categories:

  • Food, Food Service and Nutrition

Distribution Statement:

APPROVED FOR PUBLIC RELEASE