Molecular Biology of the Extremely Thermophilic Archaebacteria Methanothermus fervidus.
Annual rept. Apr 86-Mar 87,
OHIO STATE UNIV COLUMBUS DEPT OF MICROBIOLOGY
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Techniques and facilities have been developed which allow the growth of Methanothermus fervidus at 90 C using Hydrogen Carbon dioxide as the substrate for methanogenesis. Six genes which encode tRNAs, and the genes which encode the major catalyst for methane production, methyl coenzyme-M reductase, have been cloned and partially sequenced from the genome of this extremely thermophilic archaebacterium. Sequence comparisons have allowed the identification of regions of genes which are conserved in mesophiles and in the extreme thermophile. Changes identified in the genes of M. fervidus should reflect alterations needed to accommodate life at 90 C. Keywords Genetic engineering Gene cloning Microbiology.