Mass-Screening of Curarimimetic Neurotoxin Antagonists.
Annual rept. 1 Feb 86-31 Jan 87,
STATE UNIV OF NEW YORK AT STONY BROOK DEPT OF BIOCHEMISTRY
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The present search for antidotes of curarimimetic neurotoxins is based on the premise that these toxins are considerably larger than the neurotransmitter acetylcholilne, and that therefore the toxin binding site on the receptor must comprise elements other than the acetylcholine site itself. Consequently toxin antagonists can be envisaged which prevent toxin binding and action, without being themselves activators or inhibitors of the receptors. We have obtained evidence that several nontoxic small basic proteins function as such toxin antidotes in vitro. To aid in the mass screening of other antidote candidates we have shown that the DEAE cellulose disk technique, a standard receptor assay, can be adapted for large-scale screening. Preliminary results also indicate that Elisa versions of the mass screening method are feasible. Keywords neuromuscular junction.