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Dermal Influence on Epidermal Resurfacing during the Repair of Split Thickness Wounds.

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Annual rept. 15 May 82-15 May 83,

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The purpose of this study is to evaluate dermal-epidermal interrelationships during wound repair by assessing the effect on epidermal wound healing of factors derived from platelets. Two methods were used to approach the problem 1 a wound healing animal model in which partial thickness excisional wounds made on the backs of young domestic pigs were treated with platelet preparations then evaluated for epidermal resurfacing and dermal collagen biosynthesis and 2 a skin explant cultlure model in which small section of partial thickness pig skin explants were grown in medium containing fetal bovine serum andor a platelet fraction, and epidermal cell outgrowth from explant was measured. Our results are as follows 1 Whole platelets in phosphate-buffered saline do not increase dermal collagen biosynthesis in healing skin wounds 2 Thrombin-activated platelets cannot be used withthe model because of the apparent systemic effect of the thrombin on clotting time 3 Platelet homogenates 3 x 10 to the 10th power plateletsml produced a slight increase in the rate of epidermal resurfacing in healing skin 4 A commercial preparation of platelet-derived growth factor PDGF stimulated the outgrowth of epidermal cells in explant culture in the presence but not in the abcense of fetal bovine serum and 5 a platelet homogenate fraction supported epidermal cell outgrowth in explant cultures in the adsence of fetal bovine serum when added with 5 fetal bovine serum its had a net inhibitory effect on outgrowth.

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  • Medicine and Medical Research

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