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Dermal Influence on Epidermal Resurfacing during the Repair of Split Thickness Wounds.

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Annual rept. 15 May 83-15 May 84,

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The purpose of this study is to evaluate dermal-epidermal interrelationships during wound repair by assessing the effect on epidermal wound healing of factors derived from platelets. Two methods were used to approach the problem 1 a skin explant culture model in which small sections of partial thickness pig skin explants were grown in medium containing fetal bovine serum andor a platelet homogenate, and epidermal cell outgrowth form the explant was measured 2 a wound healing animal model in which partial thickness excisional wounds made on the backs of young domestic pigs were treated with platelet preparations then evaluated for epidermal resurfacing and dermal collagen biosynthesis. Our results are as follows 1 in explant cultures a Platelet Homogenate Fraction initiated cell outgrowth without serum b Platelet Homogenate Fraction stimulated the rate of outgrowth c The stimulatory factors were stable to heating at 100 C for 2 min d Commercial PDGF without serum did not support explants. With serum it had a slight stimulatory effect and e Platelet Homogenate Fraction together with serum was able to produce an inhibition of explant viability and outgrowth. 2 In wound healing a Topical application of Platelet Homogenate 5 minday for 7 days did not affect epidermal or dermal healing and b Topical application of Platelet Homogenate with Occlusion for 2 hr or 6 hr enhanced epidermal wound healing compared to Air Exposed wounds, but was not better than Vehicle with Occlusion.

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  • Medicine and Medical Research

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