A Comparative Study Regarding the Association of Alpha-2U Globulin with the Nephrotoxic Mechanism of Certain Petroleum-Based Air Force Fuels
Final rept. 1 Sep 1984-31 Aug 1986
ILLINOIS UNIV AT URBANA DEPT OF VETERINARY BIOSCIENCES
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Fischer 344 male rats have a dose and time dependent proximal tubular degeneration induced by certain petroleum-based fuels. This degeneration may be associated with a low molecular weight alpha globulin termed alpha-2U globulin . A new method was developed to obtain monospecific immunologic reagents for alpha-2U globulin using diafiltration, anion-exchange and hydroxylapatite chromatography. Rocket immunoelectrophoretic and isoelectric focusing techniques were developed to quantitatively and qualitatively assess changes in alpha-2U globulin after experimental exposure to hydrocarbon compounds. Keywords Nephrotoxicity Alpha-2u globulin Rat urinary protein Petroleum products.