Accession Number:

ADA172462

Title:

Physiology of Continuous Bone Marrow Culture Derived Permanent Granulocyte-Macrophage Progenitor Cells

Descriptive Note:

Annual rept. 1 Aug 1982-1 Aug 1983

Corporate Author:

DANA-FARBER CANCER INST BOSTON MA

Personal Author(s):

Report Date:

1983-08-01

Pagination or Media Count:

34.0

Abstract:

A careful analysis has been made of human T-cell lines for production of human Interleukin-3. The purpose of isolation of Interleukin-3 is to use it as a growth factor for unlimited self-renewal of hematopoietic progenitor cells which are capable of differentiating to mature neutrophillic granulocytes and granulocyte-macrophage progenitor cells. Several T-cell lines including K45, JURKAT, CEM, K230 have been screened for production of Interleukin-3 by assay of supernatant from cell lines for proliferation of mouse IL-3 dependent hematopoietic progenitor cell lines. IL-2 T-cell growth factor dependent human T-cell clones have been shown to produce high levels of a factor which is biologically similar to mouse IL-3. One human T-cell clone B3 has been grown to quantities of 1,000,000-10,000,000 cells and fused with a 6-thyoguanine resistant variant of K45 to produce human T-cell hybridomas has been screened for production of human IL-3. Several positive clones have been found. Supernatants shown to contain biologically active human IL-3 activity is being screened for continued self-renewal of human hematopoietic progenitor cells derived from nonadherent cells removed from long-term bone marrow cultures. In attempts to improve the longevity of human long-term bone marrow cultures for generation of the target cells for these assays, modification of our original technique of hydrocortisone addition to intraoperative marrow has been developed.

Subject Categories:

  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE