Physiology of Continuous Bone Marrow Culture Derived Permanent Granulocyte-Macrophage Progenitor Cells.
Final rept. 1 Aug 82-31 Aug 84,
DANA-FARBER CANCER INST BOSTON MA
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We have attempted to derive permanent lines of growth factor Interleukin-3 dependent permanent human hematopoietic progenitor cell lines for transfusion therapy of patients suffering from marrow suppression. This work was to parallel the work we have achieved and published previously with mouse IL-3 dependent hematopoietic progenitor cell lines. In our experiments with human continuous bone marrow cultures we attempted to derive permanent nonadherent cell lines from these cultures that would reproduce the mouse results. As a corollary to this, we attempted to derive a permanent source of human Interleukin-3 such that continuous self-renewal of cloned human IL-3 dependent cell lines would be possible. The in virto correlates we chose to study were those of in vitro phagocytosis and killing of bacteria and in vitro parameters associated with respiratory burst and physiologic parameters of human granulocytes. The results of the work over these two years have indicated that a source of human IL-3 is clearly a subset of human T-lymphocytes.
- Anatomy and Physiology
- Medicine and Medical Research