Fundamental Studies in the Molecular Basis of Laser Induced Retinal Damage.
Annual rept. Mar 79-Feb 80,
CORNELL UNIV ITHACA NY
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Tunable laser resonance Raman spectroscopy was one of the tunable laser techniques we proposed to apply to investigate the molecular basis of retinal damage problems. A principal problem in Raman spectroscopy is fluorescence which occurs at longer wavelengths lower energies than the incident laser wavelength. In photoreceptor cell preparations the principal fluorescence problem arises from accessory pigments in the pigment epithelium. Significant progress has been made in rejecting fluorescence from interfacing with the Raman spectra. This progress is based on a fundamental difference between Raman scattering and fluorescence. This difference is simply the fact that fluorescence is observed at the same spectral frequencies no matter what the incident laser excitation, whereas the Raman scattering frequencies are tied to the incident laser frequency. We proposed last year to apply such an innovation to pigmented eyes, since only then could the technique be applied with all its power to retinal damage problems at the molecular level. With this in mind, we have expended a significant effort during the past year to apply these new ideas in vivo in pigmented eyes.
- Lasers and Masers