Electron Microscopy of Intracellular Protozoa.
CASE WESTERN RESERVE UNIV CLEVELAND OHIO INST OF PATHOLOGY
Pagination or Media Count:
During this fiscal year the morphological effects are studied of an anti-trypanosomal drug, WR 163577 on Trypanosoma rhodesiense by electron microscopy. Exposure of trypomastigotes to a low concentration of the drug resulted only in condensation of kinetoplast DNA fibrils. Exposure to higher drug concentrations caused clumping of nuclear chromatin and of cytoplasmic contents. An experiment is performed on ultrastructural localization of protective antigens of Plasmodium yoelii by the use of monoclonal antibodies and ultrathin cryomicrotomy. Reported are the production of two hybridoma cell lines secreting monoclonal antibodies 25.77 and 25.1, both of which react specifically with erythrocytic merozoites of P. yoelii in the indirect immunofluorescence assay. Antibody 25.77 was reactive with a localized region within each merozoite, while antibody 25.1 appeared to be specific for the membrane of schizonts and merozoites. Both antigens induced protective immunity in mice against P. yoelii. The ultrastructure is studied of the sexual stages of P. gallinaceum during gametogenesis, fertilization, and early zygote transformation. New observations were made regarding the parasitophorous vacuole PV of gametocytes and the process of emergence in male and female gametocytes. Whereas, female gametocytes readily disrupted both the PV membrane and host cell plasmalemma during emergence, male gametocytes frequently failed to break down the plasmalemma of the host cell.