Accession Number:

ADA171458

Title:

Microwave-Induced Cataracts of the Eye Lens. Strategies for Modelling and Prevention In Vitro and In Vivo.

Descriptive Note:

Annual summary rept. 1 Jun 82-30 May 83,

Corporate Author:

UNIVERSITY OF WESTERN ONTARIO LONDON DEPT OF BIOCHEMISTRY

Personal Author(s):

Report Date:

1983-06-01

Pagination or Media Count:

23.0

Abstract:

We have developed a unique system for study of the development of microwave induced cortical cataracts of the eye lens, using as a model system the rat lens incubated in circulating tissue culture medium at controlled temperature, while being exposed to microwave irradiation. Although in unirradiated controls no cataracts occur, we have used levels of microwave irradiation at which characteristic cataractous opacities with associated globular degeneration occur. We plan to continue our studies of microwaves, using the following techniques 1 scanning and 2 transmission electron microscopy, 3 high voltage electron microscopy, a powerful technique for detecting alterations in cytoskeleton structure, 4 cryological electron-stimulated detection of x-rays EDX a technique for examining the ionic composition of fresh tissue, and 5 biochemistry. In these studies we hope to elucidate the mechanism of the previously unreported unique effects of high energy pulsed microwave, which appear to be related to thermoacoutic transduction. In the studies to date we have established several caytaractogenic does but not looked at the minimum effective dose, and have noted several features of the irradiated lens which are apparently related to the power temperature and duration of the irradiation and to the mode of delivery constant amplitude CW or high energy pulsed Pu.

Subject Categories:

  • Radiobiology
  • Stress Physiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE