Development of Serologic Assays for the Diagnosis of New World Leishmaniasis.
Annual rept. no. 1B, 1 Dec 82-30 Nov 83,
MARYLAND UNIV BALTIMORE DEPT OF PATHOLOGY
Pagination or Media Count:
Two-hundred eighty-nine monoclonal antibodies have been generated to surface and intracellular antigens of various isolates of New World Leishmania. Efforts have focused upon using these monoclonals to detect antigenic difference between Leishmania mexicana mexicana and Leishmania braziliensis panamanensis. Indirect immunofluorescent antibody assays have been used to demonstrate qualitatige differences enzyme linked immunosorbent assays have been used to demonstate quantitative differences. We appear to have at least 6 monoclonal antibodies which can distinguish between air-dried promastigotes of the two species. Additional monoclonals react with both species but the intensity of reactivity is often significantly different. In fact, we now have some degree of confidence in our ability to differentiate L.m. mexicana from L.b. panamanensis on the basis of the quantitative differences.
- Medicine and Medical Research