Human Immune Responses to Dengue Viruses.
Annual rept. Sep 82-Jul 83,
MASSACHUSETTS UNIV MEDICAL SCHOOL WORCESTER
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We are developing techniques for detecting viral-specific and non-specific lytic destruction of cells infected with dengue virus. This year we developed a persistently infected human lymphoblastoid cell line which has been used as the target cell system for detecting antibody-dependent cell mediated cytotoxicity ADCC, and to detect complement-dependent antibody mediated lysis of dengue virus infected cells. These assays presently use dengue-type 2 virus infected Raji cells as the target cells. Polyclonal hyperimmune murine sera to dengue types 2 or 4, which is type specific in neutralizing antibody assays, and convalescent human serum contain antidbodies which lyse dengue-2 infected cells in the presence of normal human peripheral blood lymphocytes in this ADCC assay. Hyperimmune polyclonal antisera to dengue 2 virus plus complement lyse dengue 2 infected cells however, antiserum to dengue 4 virus does not kill dengue 2 infected cells in the presence of complement. This apparent subtype specificity of the complement dependent lytic antibody is unlike the cross-reactive killing of infected target cells in the ADCC assay in which dengue 2 infected cells are killed by hyperimmune antisera to dengue-4 as well as to dengue-2. The lymphocyte responsible for cell lysis in the ADCC assay appears to be T3- and OKM 1.
- Anatomy and Physiology