Enzyme Mini-Test for Field Identification of Leishmania isolates from U.S. Military Personnel.
Annual rept. no. 1, 15 Mar-15 Aug 83,
YOUNGSTOWN STATE UNIV OHIO
Pagination or Media Count:
Certain WRAIR isolates have been characterized for up to 29 enzymes by cellulose acetate electrophoresis CAE and were identified as follows 6 as Leishmania braziliensis braziliensis 31 as L. b. panamensis, 3 as L. chagasi 3 as L. mexicana mexicana 1 as L. m. aristedesi 6 as diffuse cutaneous leishmaniasis 1 as L. donovani 1 as L. m. pifanoi 6 as not Leishmania 4 unknown. Allozyme polymorphism was noted among the isolates in certain of these groups, but no attempt was made at this time to associate polymorphism with any specific parameter. Culturing began July, 1983 and attempts, as yet successful, have been made to simplify the procedures followed to prepare cultures for CAE. It appears from preliminary study that certain enzymes such as, phosphogluciosomerase, GPI, mannose phosphate isomerase, MPI, and glutathione reductase, GSR, produce distinctly migrating bands for all New World isolates, and can therefore be used to accurately establish preliminary isolate identification. Preliminary studies on the enzyme systems GPI, MPI, and GSR have shown it is feasible that buffer and stain components might be packaged which eliminates the need for a mettler type balance and pH meter.