Accession Number:

ADA171377

Title:

Enzyme Mini-Test for Field Identification of Leishmania isolates from U.S. Military Personnel.

Descriptive Note:

Annual rept. no. 2, 15 Aug 83-15 Aug 84,

Corporate Author:

YOUNGSTOWN STATE UNIV OHIO

Personal Author(s):

Report Date:

1984-08-15

Pagination or Media Count:

36.0

Abstract:

It is possible to identify Leishmania isolates by cellulose acetate electrophoresis CAE of up to 29 enzyme activities. Certain of these enzymes are polymorphic within a subspecies and therefore of limited value for identification others are monomorphic and have taxonomic significance. Once large numbers of isolates from various geographical areas have been characterized and monomorphic enzymes identified, a simple, rapid, accurate field type identification test can be devised. Approximately 200 Leishmania isolates have been characterized for up to 29 enzymes by CAE. Enzymes profiles based on reference strain isolates have been established by L. braziliensis panamensis, L. b braziliensis, L. b guyanensis, L. mexicana mexicana, L. m. aristedesi, L. pifanoi, L. m. enriettii, L. garnhami, L. donovani, L. chagasi, L. major, L. tropica, L. aethiopica, and L. hertigi hertigi. It is possible that specific enzyme polymorphism is related to drug susceptibility. The test leading to accurate and rapid identification of Leishmania isolates requires analysis of enzymes which produce distinctly migrating bands for each subspecies, are monomorphic and are simple to run. Enzymes which appear to meet these requirements are GOT, GPI, GSR1, GSR2, ICE, MDH, MPI and 6PGDH.

Subject Categories:

  • Biochemistry
  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE