Pseudomonas flagella Vaccine in Burns.
Annual summary rept. 15 Jul 82-14 Jul 83,
TENNESSEE UNIV KNOXVILLE DEPT OF MICROBIOLOGY
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This research investigated a Pseudomonas flagellar antigen FAg as a possible vaccine for prevention of Pseudomonas infections of wounds, burns and trauma associated infections. A second aim was directed toward understanding the interaction of various Pseudomonads with the host with respect to the role of motility in colonization, invasion, and protection by a FAg induced immune system. Purification of the FAg has centered on reducing the level of LPS the major contaminant in the final material. Some progress was made using a column-detergent method in conjunction with large fermentation facilities developed in collaboration with Immuno Co. We continue to explore and improve techniques of culturing the organism to reduce LPS and contaminating protein in the initial medium. Alternative approaches are described to find more efficient purification procedures. Our knowledge of the Pseudomonas aeruginosa a and b FAg types has been expanded significantly by creating a system of classification based on flagellin molecular weights which remains consistent with immunologic determinations and in preliminary experiments by protective capacity. A taxonomic correlation was found to exist between Flagellin molecular weights of various Pseudomonas spp. and their antigenic relationships.