An Investigation of the Memory Response of the Local Immune System to Shigella Antigens.
Annual rept. 1 Feb 83-31 Jan 84,
MICHIGAN UNIV ANN ARBOR
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Recently, our laboratory has shown that by immunizing animals orally with live, locally invasive or noninvasive shigella significant enhancement in the local IgA response is consistently achieved to subsequent oral challenges with live invasive shigella simulating the time course of a natural infection 14-16. Therefore, we have hypothesized that the mucosal route of vaccination against dysentery and other primary enteropathogenic infections can be accomplished by using an oral vaccine with the appropriate antigen. In the past year and a half, we have explored the key variables in the stimulation of such mucosal immune responses and most recently are determining the functional significance of having achieved a vigorous antigen-specific local immune response. The presently reported studies include exploration of the initial processing of macromolecules in the lumen by M cells overlying isolated lymphoid follicles, the immunogenic capabilities of killed shigella antigen preparations when presented directly to the gut-associated lymphoid tissues, the effect of parenteral adjuvants on shigella-specific local IgA responses and their enhancement by concurrent local antigen administration, and functional in vivo and in vitro studies demonstrating that shigella-specific secretory IgA can collaborate with intra-epithelial lymphocytes to protect against this enteropathogen.
- Medicine and Medical Research