Distribution and Characterization of Antigens Found in Subcellular Fractions of African Trypanosomes.
MIAMI UNIV FLA DEPT OF MICROBIOLOGY
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In the previous report, the results of cell fractionation experiments of bloodstage forms of the African trypanosome Trypanosoma rhodesiense together with various forms of immuno-electrophoresis, revealed the principal antigenic sites to be associated with the surface and flagella pocket membranes. For the period of this report the investigations described were firstly concerned with utilizing the information referenced above to permit the preparative isolation of both these membrane fractions. Having achieved that objective it was then possible to assess various procedures for the isolation of antigenic components from both membrane fractions with a comparison of their properties. For this purpose attempts were made both at dissociating intact antigenic components from labelled membranes, through the use of detergents, or releasing exposed moieties by means of limited peptidolysis. The usefulness of various chromatographic procedures could then be appraised as a means of separating and characterizing specific membrane antigens. The other aspect that has been studied is the importance of membrane lipid in the binding of antigens and whether any of these antigens are associated with integral membrane protein. This was of interest in view of the published evidence for a hydrophobic c terminal portion on the variable surface antigen that is believed to penetrate the surface membrane lipid bilayer.
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