Correlation of Mutagenic, Carcinogenic and Cocarcinogenic Effects of Chemical Substances. Granuloma Pouch Assay
Final rept. 1 Sep 1982-31 Aug 1985
EIDGENOESSISCHE TECHNISCHE HOCHSCHULE AND ZURICH UNIV SCHWERZENBACH (SWITZERLAND) INST OF TOXICOLOGY
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The research project is concerned with the detection of premalignant and malignant cells induced in vivo in a novel assay system the granuloma pouch assay. Cells exposed to carcinogens in vivo can be studied for DNA damage, chromosomal aberrations, specific locus mutations and cell transformations. Various assays were developed to investigate the growth characteristics of normal, carcinogen-exposed and transformed granuloma pouch cells. These included primary cloning efficiencies under optimal growth conditions, growth in serum deficient and calcium depleted media, growth in soft agar, growth in athymic mice and rats, growth with cocultured normal cells, growth under influence of growth factors. Phenotypic alterations investigated were the DNA- dispersion in individual cells and the appearance of calcium binding proteins. Furthermore the genotoxiccytotoxic activity of asbestos fibers was investigated.
- Medicine and Medical Research