Studies of the Outer Membrane Proteins of Campylobacter Jejuni for Vaccine Development.
Final rept. 1 Sep 82-30 Nov 83,
COLORADO UNIV HEALTH SCIENCES CENTER DENVER
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The major objective of our fifteen months of study was to develop methods for isolating outer membrane-enriched fractions of Campylobacter jejuni. Using standard disruption and solubilization techniques, we have prepared outer membrane-enriched fractions of C. jejuni and have characterized the proteins resolved. Subsequently, we began studies to determine which are antigenic and thus could have potential as vaccine candidates. Using both radioimmunoprecipitation and immunoblot procedures, we have acquired preliminary data on the antigenicity of these proteins. Currently, we have continued to explore the feasibility of using mice for an animal model of Campylobacter infection in which to test vaccine candidates. After original characterization of the consequences of oral challenge, we have focused on determining the kinetics of the bacteremia produced, and the mechanisms for clearance. We have also developed an enzyme-linked immunosorbent assay ELISA for determining human IgA, IgG, and IgM responses to C. jejuni surface antigens. This technique will be of value at the time human subjects are challenged with candidate vaccines.
- Medicine and Medical Research