Nerve Regeneration in vitro: Comparative Effects of Direct and Induced Current and NGF.
Final rept. 1 Jan 82-31 Dec 84,
KENTUCKY UNIV RESEARCH FOUNDATION LEXINGTON
Pagination or Media Count:
This investigation focused on the response of sensory neurons DRG obtained from young 8 day and older 14 day chick embryos and 15 day old rat fetuses to low levels of direct DC or induced PEMF current or NGF. Direct current 10 nA was applied continuously for 3 days through tantalum electrodes immersed in the media current was induced by exposing cultures to pulsed electromagnetic fields PEMF using a single pulse, 72 Hz, 12 hrsday for 2 days with coils supplied by Electrobiology Inc. The data presented in the Appendix indicates the following in 8 day cultures, the growth response obtained with NGF, PEMF or DC was significantly increased over controls at 3 days and this was maintained only with NGF or DC by 6 days. PEMF effects were correlated with current density only when the coils were oriented vertically. Significantly-increased protein content and incorporation of 3H-prolineganglia paralleled the increased growth response obtained with DC. In older 14 day DRG, neurite outgrowth was enhanced only with DC. The growth response in 15 day fetal DRG was not influenced by PEMF or DC only NGF stimulated growth. Neurophysiological studies found the resting potential of the DRG neurons to range from 30-60 mV they exhibited overshooting potentials upon intracellular stimulation. Equivocal results were found in membrane potential changes when DC was applied.
- Anatomy and Physiology