A Reliable Method for Recovering Cryopreserved Hybridoma Cells for Cloning,
ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD
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A method is described for the reliable recovery of hybridoma cell lines from cryogenic storage. This procedure provides for a the efficient retrieval of cells secreting antibody for rapid confirmation of specific antibody production b cells for cloning c cells for restocking the liquid nitrogen storage banks and d cultures to detect and possibly eliminate contamination.
- Medicine and Medical Research