Accession Number:

ADA170497

Title:

A Reliable Method for Recovering Cryopreserved Hybridoma Cells for Cloning,

Descriptive Note:

Corporate Author:

ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD

Report Date:

1985-01-01

Pagination or Media Count:

3.0

Abstract:

A method is described for the reliable recovery of hybridoma cell lines from cryogenic storage. This procedure provides for a the efficient retrieval of cells secreting antibody for rapid confirmation of specific antibody production b cells for cloning c cells for restocking the liquid nitrogen storage banks and d cultures to detect and possibly eliminate contamination.

Subject Categories:

  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE