Human Y-79 Retinoblastoma Cells Exhibit Specific Insulin Receptors,
ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD
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The presence of insulin receptors was in investigated in human Y-79 retinobastoma cells grown in suspension culture. The binding of 125I insulin to these cells was time, temperature, and pH dependent, was competed for by insulin and proinsulin but not other peptides, and was inhibited by antibodies against the insulin receptor. Negative cooperativity was not found, in agreement with other studies in rodent neural cells. However, in contrast to studies with rodent cells, insulin specifically down-regulated its receptor on human Y-79 cells after prolonged exposure. In conclusion, these data show for the first time the presence of specific insulin receptors in human Y-79 retinoblastoma cells. Because these cells were previously shown to have several characteristics typical of neural cells, we propose their use as a model to study the effects of insulin on neural and retinal tissues of human origin.
- Medicine and Medical Research