Absence of Mutagenic Activity of Hycanthone in Serratia marcescens,
ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD
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Hycanthone methanesulfonate, an antischistosomal agent, is an effective frameshift mutagen in Salmonella typhimurium but did not induce auxotrophic mutations in Serratia marcescens HY, even at high concentrations 25,000 ugml. The mutagenic action of hycanthone in S. typhimurium is not dependent on a functional excision repair system but is enhanced by the plasmid pKM101, which mediates the inducible error-prone repair system. Hycanthone, like proflavin, intercalates between the stacked bases of DNA. Upon replication, DNA lesions are generated which induce error-prone repair. It is suggested that s. marcescens HY is insensitive to the mutagenic action of hycanthone because it lacks an error-prone repair system.