An Investigation into the Effects of Peptide Neurotransmitters and Intracellular Second Messengers in Rat Central Neurons in Culture.
Annual rept. 15 Oct 84-15 Oct 85,
AT AND T BELL LABS INC MURRAY HILL NJ
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Significant progress has been made in several areas of our research proposal. We have developed a Ca-imaging system which is now capable of resolving subcellular Ca changes on the order of seconds. This Ca-imaging system has been used to study neurotransmitter actions in culture diencephalic and cerebellar neurons. Both classical GABA and modulatory thyroid hormone neurotransmitters were found to affect cellular Ca2 levels in these cells without depolarizing resting potential. We have also continued studies of second messenger action in identifiable molluscan neurons. The concentration of endogenous cyclic AMP in single cells was measured, and under stimulated conditions the levels were sufficient to suggest activation of cyclic AMP-induced membrane conductances.
- Anatomy and Physiology