Actions of Interferons on Macrophages.
Final rept. May 85-Jun 86,
TRUDEAU INST INC SARANAC LAKE NY
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A rat anti-murine gamma interferon MuIFN gamma neutralizing monoclonal antibody MAB was used ot investigate the effect of MuIFN gamma on te development of antiviral resistance, Fc receptor expression, and Ia antigen in macrophages. Theses studies were carried out in cultures f C3HHeJ peritoneal macrophage cultures in order to determine 1 whether MuIFN gamma can influence the expression of the above parameters, 2 the relative quantity of MuIFN gamma required to mediate each effect, and 3 the amount of anti-MuIFN gamma MAb required to neutralize each MuIFN gamma-mediated effect. The results of these studies established that macrophages are at least as sensitive to the antiviral action of MuIFN gamma as other cell types tested, however, substantially more MAb was needed to neutralize MuIFN gamma-mediated antiviral activity in macrophages. It was also found that 10-15 times more MAb was required to inhibit MuIFN gamma induced Fe receptor expression than required for Ia antigen expression. These later findings confirm and extend previous observations, which indicate the MuIFN gamma induction of enhanced expression of these two macrophage membrane differentiation markers occurs through distinct mechanisms. During the course of a sublethal Listeria monocytogenes infection, all three IFN classes alpha, Beta, gamma are produced by the host. In addition to their antiviral activities, IFNs can mediate a variety of different effects which could conceivably have a bering on the expression of host nonspecific andor specific resistance mechanisms.
- Medicine and Medical Research