Structure of Antibodies by X-Ray Diffraction of Monomolecular Layers and by Nuclear Magnetic Resonance.
STANFORD UNIV CA STAUFFER LAB FOR PHYSICAL CHEMISTRY
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This contract research has demonstrated that a remarkably large amount of information about the combining site structure of a monoclonal anti-spin label paramagnetic free radical antibody can be determined from the NMR spectrum of this protein. This opens the way to the study of structural changes in the combining site due to somatic cell mutations small numbers of amino acid substitutions. This work is now in progress. The initial phases of this work were directed towards applying and developing the technique of surface x-ray diffraction for the study of antibody structure. During the course of the contract, there was a significant change, not in objective antibody structure but in our approach to this problem NMR rather than x-ray diffraction. This change was brough about by two factors 1 In addition to a number of difficult technical problems that were encountered, the beam time available to us per calendar year at the Stanford Synchrotron Radiation Laboratory SSRL proved to be too short to permit significant progress. 2 We found that the NMR spectra of a monoclonal anti-spin label antibody provide a remarkably powerful and much more rapid approach to this problem. Both lines of research are summarized.
- Atomic and Molecular Physics and Spectroscopy