Accession Number:

ADA167921

Title:

Semiconducting Chemical Microsensors Using Bioanalogous Principles.

Descriptive Note:

Final rept. 1 Oct 82-28 Feb 86,

Corporate Author:

MICHIGAN STATE UNIV EAST LANSING COLL OF NATURAL SCIENCE

Personal Author(s):

Report Date:

1986-04-24

Pagination or Media Count:

31.0

Abstract:

The objectives of our research on the d.c. conductivity of proteins was to investigate and demonstrate that appropriate electrical properties of macromolecular substrates of biological origin can be exploited for analytical purposes using macroscopic techniques. More concretely, we wanted to show that the specificity and selectivity of specific antibodies in molecular target recognition and binding, combined with monitoring binding-induced changes in their d.c. electric conductivity may represent such properties. If so, at least these bimolecular systems can be considered as potential sensory elements in designing chemically highly selective and very sensitive chemical microsensors. Such chemically sensitive gate elements of appropriate bimolecules in Field Effect Transistor - type devices could then be ued for the real-time evaluation of vanishingly smal quantities of diverse molecular species present in the vapor gas or liquid form. Our investigations demonstrated that the utility of d.c. conductivity measurements on thin immunoprotein films as a probe of antigenhaplen binding by monoclonal move anti-DNP IqE antibody. Upon binding to the antibody, appropriate nitropenyl ligands 2,4-DNP-Lysine, TNP bring about typical changes in the d.c. conductivity it decreases by about a factor of two. Model compounds BSA and its derivatived forms were used to provide information about the possible mechanism of resistance lowering by DNP-based haptens.

Subject Categories:

  • Biochemistry
  • Electricity and Magnetism
  • Solid State Physics

Distribution Statement:

APPROVED FOR PUBLIC RELEASE