Tricothecenes Mycotoxin Studies.
Annual summary rept. Jun 83-Sep 84,
MASSACHUSETTS INST OF TECH CAMBRIDGE
Pagination or Media Count:
The purposes of the research supported under this contract are to determine the toxicity of two members of the tricothercene mycotoxins, anguidine, DAS and nivalenol including their gross and microscopic effects absorption, distribution and excretion by several routes of administration to identify and synthesize the more prominent metabolites, and to identify or synthesize compounds which will block or reduce the toxicity of DAS and nivalenol. During the past year we have developed a method for topical exposure to the mycotoxins in rodents and in guinea pigs which confirms our observations by other routes of administration that there is a significant difference in sensitivity between rats and mice. The mouse excretes 80 of a dose of DAS within 24 hours, 90 of which appears in the urine. This is in contrast to most literature reports on other tricothecenes T-2 where the feces is the major route of excretion. We have developed a system colony forming unit to study stem cells of the bone marrow of rodents exposed to DAS. In the rodent the tests is the first and most sensitive organ as an indicator of DAS toxicity and is being used to detect earliest effects and correlate these with metabolites in animals exposed by different routes of exposure including mice, rats and guinea pigs, the latter by the respiratory route.