Accession Number:

ADA167378

Title:

Characterization of Antibody-Dependent Killing of Trypanosomes by Macrophages.

Descriptive Note:

Final rept. 15 Sep 81-14 Nov 83,

Corporate Author:

ALBERT EINSTEIN COLL OF MEDICINE BRONX NY

Report Date:

1985-01-01

Pagination or Media Count:

38.0

Abstract:

In the presence of specific antibody, murine peritoneal macrophages and macrophage-like cell lines bind Trypanosoma rhodesiense parasites in vitro. Using a large bank of anti-WRATat 1.1 specific monoclonal antibodies, a high correlation was found between the ability of these products to neutralize trypanosome infection in vivo and mediate binding of trypanosomes to macrophages in vitro. There was also a high coincidence between function in the macrophage-binding assay and the ability of a monoclonal antibody to bind trypanosomes in an indirect immunofluoresence assay. The mechanism by which trypanosomes penetrate macrophages was also investigated. The data suggest that these organisms do not actively penetrate macrophages, but instead are phagocytized by the macrophages. The mechanism underlying the enhancement of binding by fresh serum was analyzed, and the C3 component of complement was found to be important. Possible generic control of macrophage function was also investigated. Normal peritoneal macrophages from C57BL6, BALBc, and C3HHeJ mice do not differ in their trypanosome-binding ability. However, 10 days post-infection, cells from genetically susceptible, BALBc mice bound more trypanosomes that did those from infected genetically resistant, infected C57BL6 mice. A number of macrophage cell lines were analyzed for their trypanosome binding ability.

Subject Categories:

  • Biochemistry
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE