Ultrasensitive Detection of Chemical Substances.
Quarterly rept. 1 Sep-30 Nov 85,
NEW MEXICO UNIV ALBUQUERQUE SCHOOL OF MEDICINE
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The past quarter focused on Rivanol studies. For some time we have worked to enhance binding of trapping agents, proteins, to surfaces and at the same time reduce non-specific binding or noise. Another concern has been to bind trapping agents so that hey would remain in an active configuration. Our discovery that Rivanol, an agent used in the precipitation of antibodies, enhances protein binding to polystyrene lead us to investigate its possible use as a binding agent. Our study of Rivanol has had many stumbling blocks, however after an intensive study we are now taking steps to apply for a patent covering the use of Rivanol as a binding agent. This report covers the salient points of our work with Rivanol. Some of these results appear in previous reports. Other acridines also increase binding of proteins to polystyrene. Quinicrine, acridine orange, 9-amino acridine, acriflavin and Rivanol increase the binding of eel acetylcholinesterase to polystyrene, albeit at different efficiencies. Currently, we are continuing to examine the efficiency of Rivanol in binding proteins, both in pure form and in serum, to polystyrene.