Accession Number:

ADA164603

Title:

Multiple Effects of Immunological Adjuvants on Lymphatic Microenvironments. I. Role of Immunologically Relevant Angiogenesis in the Mechanisms of Action of CFA, MDP and Avridine,

Descriptive Note:

Corporate Author:

ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD

Personal Author(s):

Report Date:

1985-01-01

Pagination or Media Count:

11.0

Abstract:

Complete Freunds adjuvant CFA, muramyl dipeptide MDP in oil, and the lipoidal amine Avridine, exhibit comparable adjuvant activities with regard to cellular and humoral immune responses to viral antigens when administered parenterally with antigen. The present study correlates the ability of the above agents to produce alterations in the lymphatic microenvironments of the local injection site and regional lymph node with enhancement of immunity. These adjuvants increase lymphoid and mononuclear cell traffic in the local site and regional lymph nodes, initially by effecting blood flow and transvascular chemotaxis, and subsequently by inducing angiogenesis and differentiation of specialized high-endothelial venules. The development of lymphatic tissue in the local injection site corresponds chronologically with the appearance of precursors of monocytes, interdigitating dendritic cells IDC, fibroblastic reticular cells FRC, and vascular endothelial cells in thoracic duct lymph. Thus effective adjuvants are capable of causing systemic alterations to lymphatic microenvironments through mobilization of various accessory cell types which may metastasize to resolving sites of inflammation. In addition, culture supernatants from these adherent cell types are effective in vitro as stimuli for lymphocyte chemotaxis, lymphocyte activation and endothelial cell proliferation. In conclusion, immunological adjuvants appear to be exceedingly complex in their mechanisms for enhancing the functions of the lymphatic system.

Subject Categories:

  • Medicine and Medical Research
  • Pharmacology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE