Airborne Induced Experimental Bordetella bronchiseptica Pneumonia in Strain 13 Guinea Pigs
Infections Study Sep-Nov 1984
ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD
Pagination or Media Count:
Strain 13 guinea pigs were evaluated as an animal model to study the molecular interactions of Bordetella sp and to evaluate the efficacy of B. bronchiseptica bacterins in inducing a protective immune response. When guinea pigs were exposed to small particle aerosols of varying concentrations of virulent B. bronchiseptica, a spectrum of disease was produced that ranged from inapparent to fulminant. Clinical signs began by day 4 after exposure, and were evidenced by anorexia, weight loss, respiratory distress, and serous to purulent nasal discharge. Pathologic alterations were limited to the respiratory system. Moribund animals exhibited a suppurative, necrotizing bronchopneumonia and necrotizing tracheitis. Typically, the bacteria were eliminated from the lungs by day 28, while significant concentrations of bacteria continued to persist in the laryngeal area and in the trachea. The median infections dose ID50 and the median lethal dose LD50 were estimated to be 4 CFU and 1,314 CFU, respectively. These data suggest the guinea pig will be a valuable model system in which to study Bordetella sp-host cell interactions, as well as evaluate potential B. bronchiseptica immunogens.
- Anatomy and Physiology
- Medicine and Medical Research