Trypanosoma b. Rhodesiense (WRATat Serodeme): Purfication and Characterization of Surface Antigens for the Vaccine Development Program.
Annual rept. no. 2 (Final) Jul 79-Aug 81,
COLORADO STATE UNIV FORT COLLINS COLL OF VETERINARY MEDICINE AND BIOMEDICAL SCIENCES
Pagination or Media Count:
The variant-specific glycoproteins VSGs from some of the antigenic clones of organisms of the Trypanosoma b. rhodesiense WRATat serodeme were purified from cell lysates. Pooled eluate-fractions of lectin-affinity chromatography using concanavalin-A agarose was the primary fractionation tool followed by gel permeation chromatography on TSK, SW-3000 HPLC columns. VSGs which showed single band purity by denaturing polyacrylamide gel electrophoresis were hydrolized with 3N mercaptoethanesulfonic acid and their amino acid compositions determined. Studies were initiated to determine how to manipulate the rats and the parasites to obtain better and consistent yields of trypanosomes from the blood of the infected animals. Host responses to acute infections were measured for changes in glucose, insulin, multiplication-stimulating activity MSA, nonsuppressible insulin-like activity NSILA, lactate, catecholamines and others. Attempts were made to isolate and clone acute, isoantigenic trypanosomes from the terminal waves of parasitemia initiated with one of the chronic clones of T.b. rhodesiense WRATat-5. An acute strain was isolated, cloned and designated T.b. rhodesiense CSUT-J1.
- CHEMICAL COMPOSITION
- INFECTIOUS DISEASES
- AMINO ACIDS
- Medicine and Medical Research