Animal Models for the Prevention of Acute and Chronic Graft-vs-Host Disease.
Annual summary rept. Sep 81-Sep 82,
CITY OF HOPE NATIONAL MEDICAL CENTER DUARTE CA
Pagination or Media Count:
One of the purposes of the work was to develop a better understanding of the mechanism whereby pre-treatment of a mixture of bone marrow and spleen cells which are used in a murine model of bone marrow transplantation with rabbit antisera against murine lymph node tissue could effect not only acute graft-versus-host disease mortality but also chronic graft-versus-host disease mortality. The second major objective of the research was to evaluate monoclonal antibodies to several T cell differentiation antigens both as agents for the in vitro pretreatment of bone marrow and spleen cell grafts to modify their graft-versus-host disease potential, and also as agents for the in vivo treatment of established graft-versus-host disease. The methods used involved a well characterized murine model of bone marrow transplantation in which recipient mice are treated with lethal doses of total body irradiation and reconstituted with allogeneic bone marrow cells or a combination of allogeneic bone marrow and spleen cells. Monoclonal antibodies against T-cell differentiation antigens were used to treat the in vitro cells prior to transplantation in order to eliminate T-cells. The monoclonal antibodies were also used in in vivo experiments in an effort to influence the course or established graft-versus-host disease.
- Anatomy and Physiology
- Medicine and Medical Research