Dependence of Endothelial Cell Prostacyclin Synthesis on Cell Shape and F-Actin Cytoskeletal Arrangement,
ARMY RESEARCH INST OF ENVIRONMENTAL MEDICINE NATICK MA
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Alterations in endothelial cell shape, as measured by the planar surface area encompassed by the cell perimeter adhering to the substrate, were associated with modulation of basal prostacyclin synthesis. Cells with small surface area generated the highest levels of prostacyclin and had both a diffuse arrangement of F-actin and an increased binding capacity for rhodamine-phalloidin. Large cells with many actin cables and a significantly reduced phalloidin binding capacity produced significantly smaller quantities of prostacyclin. These findings suggest a cytoskeletal role in the regulation of prostacyclin production and more specifically, that changes in F-actin arrangement modulate basal endoperoxide metabolism.
- Anatomy and Physiology