Characterization of Immune Suppression Induced by Polyribonucleotides.
Final rept. Aug 82-Nov 85,
MINNESOTA UNIV DULUTH DEPT OF MEDICAL MICROBIOLOGY AND IMMUNOLOGY
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Polyribonucleotide complexes, which have been shown to be potent adjuvants to the helperinducer cells of the immune response of animals and humans were tested for their capacity to activate also cells involved in suppressing antibody synthesis. Poly A-poly U and poly I-poly C were found to inhibit murine antibody forming cells when given 1-6 days before antigenic stimulus. Attempts to identify the cell population responsible for the suppression revealed it could be transferred on day 2 after antigen with preparations enriched for both T or B cells as well as adherent cells. Thus, multiple cells may each contribute to the total suppression or a single cell type may be contaminating each of the isolated populations. Since the natural killer NK cell population has been shown by others to suppress the immune response, this likely candidate was rendered non-functional with anti-asialo antiserum with, however, no diminution in suppressive activity. Further experiments are required to define this adjuvant induced non-specific suppressor cell. Activation of suppression by the polynucleotides did not diminish murine resistance to challenge with either Streptococcus pneumoniae or Pseudomonas aeruginosa.
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