Japanese Encephalitis Virus Immunoglobulin M Antibodies in Porcine Sera
ARMED FORCES RESEARCH INST OF MEDICAL SCIENCES BANGKOK (THAILAND)
Pagination or Media Count:
A solid-phase enzyme-linked immunosorbent assay ELISA was developed for detection of porcine immunoglobulin IgM antibodies to Japanese encephalitis virus JEV. Antibodies in sera were captured onto the solid phase of Microtiter plates sensitized with mouse monoclonal antibodies to porcine u heavy chain. Virus antigen binding to the lawn of IgM as quantitated by subsequent binding of peroxidase-labeled human hyperimmune anti-JEV IgG, which, in the final step, catalyzed a substrate color change.
- Medicine and Medical Research