Accession Number:

ADA162267

Title:

Japanese Encephalitis Virus Immunoglobulin M Antibodies in Porcine Sera

Descriptive Note:

Journal article

Corporate Author:

ARMED FORCES RESEARCH INST OF MEDICAL SCIENCES BANGKOK (THAILAND)

Report Date:

1985-10-01

Pagination or Media Count:

5.0

Abstract:

A solid-phase enzyme-linked immunosorbent assay ELISA was developed for detection of porcine immunoglobulin IgM antibodies to Japanese encephalitis virus JEV. Antibodies in sera were captured onto the solid phase of Microtiter plates sensitized with mouse monoclonal antibodies to porcine u heavy chain. Virus antigen binding to the lawn of IgM as quantitated by subsequent binding of peroxidase-labeled human hyperimmune anti-JEV IgG, which, in the final step, catalyzed a substrate color change.

Subject Categories:

  • Medicine and Medical Research
  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE