Decreased Wound Neutrophils and Indiscrete Margination in the Pathogenesis of Wound Infection
ARMY INST OF SURGICAL RESEARCH FORT SAMHOUSTON TX
Pagination or Media Count:
To assess the pathogenesis of increased susceptibility to infection and septic death in a rat model, netrophils PMNs in the wound, circulating PMNs, and their in vivo activity were evaluated after 30 and 60 of burns. Eight hours after injury there were twice as many PMNs in the wounds of rats that sustained 30 compared with 60 burns. There was no difference between these two groups in the number of circulating PMNs at 2,4,6, and 8 hours of sleep after injury. In vivo evaluation of PMN response to infusion of F-Met-Leu- Phe revealed that circulating PMNs were more sensitive 4 hours after 60 burns compared with sham burns. At this time PMNs were found to be less sensitive to zymosan-activated serum infusion after 30 burns were more sensitive to this stimulus than were PMNs in rats with 60 injuries. Thus rats with greater injury, known to be more susceptible to wound infection, have fewer PMNs in their wounds 8 hours after injury. This is preceded by an increased sensitivity of PMNs in vivo to bacterical chemotactic factor and a relative increase in sensitivity to wound factors. This unusual finding implicates indiscrete margination as a factor in the pathogenesis of infection.
- Medicine and Medical Research