Potential for Cross-Reactive Protection Using Peptides and Adjuvants or Carrier Molecules
Annual rept. 1 May 1984-30 Apr 1985
MASSACHUSETTS UNIV MEDICAL SCHOOL WORCESTER DEPT OF MEDICINE
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We have demonstrated that a conserved portion of the HA2 subunit on the influenza virus hemagglutinin can induce a cytotoxic T lymphocyte response. This is a major development since it raises the possibility that this type of peptide could be used to provide protection that would be cross-reactive among influenza virus strains. The peptide we used was produced in E. coli using recombinant DNA techniques for the expression of segments of influenza viral genome. The molecule which stimulates this H-2 restricted cytotoxic T lymphocyte response is a fusion protein of the HA2 subunit of H1 virus APR834 H1N1, and the induced lymphocytes kill target cells infected with strains of influenza A virus possessing the H1 hemagglutinin irregardless of the years isolated e.g. 1934, 1978, the results indicate that the HA2 subunit is a candidate for cross- reactive protection because there are substantial published data indicating that influenza virus induced cytotoxic T lymphocytes Tc are protective in challenged recipients. Originator supplied keywords include Lymphokines and Epitopes.
- Medicine and Medical Research