Accession Number:

ADA160059

Title:

Clonal Analysis of HLA-DR and -DQ Associated Determinants - Their Contribution to Dw Specificities,

Descriptive Note:

Corporate Author:

MINNESOTA UNIV MINNEAPOLIS

Personal Author(s):

Report Date:

1985-01-01

Pagination or Media Count:

39.0

Abstract:

In order to investigate the distribution of epitopes recognized by T cell clones directed against HLA class II products, bulk primed cell populations were generated using cells matched for class I determinants but disparate for class II determinants. Cells were cloned by single cell deposition FACS IV or limiting dilution 1 cell3 wells, and assayed for proliferative and cytolytic function with panels of well-characterized cells. Our data indicate that determinants detected on both DR and DQ products are associated with the Dw type of the sensitizing cell showing that there is polymorphism recognized by T cells on both DR and DQ that is subtypic to the serologically defined specificities. Thus, it appears that the bulk T cell response is a composite of individual clones recognizing distinct determinants on these class II molecules. The implications of these findings for studies of HLA restricted recognition are discussed.

Subject Categories:

  • Anatomy and Physiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE